Numbered runs
Three runs of the same published protocol. Each step shows what was planned, the value recorded and the observation written down. A value outside the allowed window is marked as a deviation.
Deviations by step and run
| Step | Run 1 | Run 2 | Run 3 |
|---|---|---|---|
| 1. Thaw and stage reagents | |||
| 2. Dispense sample and mix A | |||
| 3. Incubate | |||
| 4. Thermal cycling | |||
| 5. Clean-up | |||
| 6. Measure concentration | |||
| 7. Pool and load | not reached |
CompletedStarted 2026-09-08Operator A0 deviations
Step 1: Thaw and stage reagentsPrepare
- Thaw time
- Planned 20 min, recorded 20 min
Observation: Reagents clear, no precipitate.
Step 2: Dispense sample and mix ADispense
- Volume per well
- Planned 25 uL, recorded 25 uL
Observation: All 24 wells dispensed.
Step 3: IncubateIncubate
- Temperature
- Planned 37 C, recorded 37 C
- Duration
- Planned 30 min, recorded 30 min
Observation: Block held steady.
Step 4: Thermal cyclingThermal cycle
- Cycles
- Planned 12 cycles, recorded 12 cycles
Observation: Cycle completed without alerts.
Step 5: Clean-upClean-up
- Bead ratio
- Planned 0.8 x, recorded 0.8 x
Observation: Beads resuspended cleanly.
Step 6: Measure concentrationMeasure
- Mean concentration
- Planned 4 ng/uL, recorded 4.3 ng/uL
Observation: Readings consistent across the plate.
Step 7: Pool and loadPool and load
- Loading amount
- Planned 12 pM, recorded 12 pM
Observation: Pool loaded, no bubbles.